Plasmids and Cultures

This database contains entries for 3,572 known and predicted gene coding sequences in Synechocystis sp PCC 6803 based on the Kazusa genome sequence and nomenclature (http://genome.microbedb.jp/cyanobase/GCA_000009725.1). Plasmids for generating marked mutants and mutant strains can be searched for by gene Name/Accession (e.g. 'sll1226' or '1513_sll1226-sacB'),  gene homology (e.g. 'hydrogenase large subunit'), Gene ID (e.g. hoxH), or by a portion of the gene coding sequence Sequence (CDS).

Please note, the library is still being completed. Plasmids and/or marked mutants may not be available. Users can also search for available materials by Plasmids made or Mutant made.

Plasmids, mutants and the unmarking spacer plasmid pUC19K-UL are currently available on request. Regardless of the segregation status of the mutant, open reception of a cyanobacterial mutant strain we recommend 1-2 cycles of restreaking on a selection plate containing kanamycin (100 μg/ml).