Plasmids and Cultures

This database contains entries for 3,572 known and predicted gene coding sequences in Synechocystis sp PCC 6803 based on the Kazusa genome sequence and nomenclature (http://genome.microbedb.jp/cyanobase/GCA_000009725.1). Plasmids for generating marked mutants and mutant strains can be searched for by gene Name/Accession (e.g. 'sll1226' or '1513_sll1226-sacB'),  gene homology (e.g. 'hydrogenase large subunit'), Gene ID (e.g. hoxH), or by a portion of the gene coding sequence Sequence (CDS).

Please note, the library is still being completed. Plasmids and/or marked mutants may not be available. Users can also search for available materials by Plasmids made or Mutant made.

Plasmids, mutants and the unmarking spacer plasmid pUC19K-UL are currently available on request. Regardless of the segregation status of the mutant, open reception of a cyanobacterial mutant strain we recommend 1-2 cycles of restreaking on a selection plate containing kanamycin (100 μg/ml).

Name Sort descending CDS Gene Homology Gene ID
1401_sll1985-sacB ATGAAATTATCCGTTCTTACTGTGG… transposase
1402_sll1984-sacB ATGTTAAGAGAACTAATTAAGCTCT… transposase
1403_sll1983-sacB ATGGCTTACAGTTTAGATTTAAGGC… transposase
1404_sll1982-sacB GTGGGTAGGATAGAATGGAGGAAAA… transposase
1405_slr2092-sacB ATGGCTAGGTCTTTGGGCAGAACGC… Uma2 family endonuclease
1406_sll1981-sacB ATGGGGCAAATGAACACCGCAGACC… Glyoxylate carboligase; acetolactate synthase large subunit ilvB
1407_ssl3769-sacB ATGTTGGTACTTTCCGTCCTATCTT… hypothetical protein
1408_sll1980-sacB ATGACCCCGGCCAAAATTCGTAACG… thiol:disulfide interchange protein; TrxA trxA
1409_sll1979-sacB ATGACTGAGTCCGTAATCTCCCCAG… Cyanobacterial Signature Protein
1410_slr2094-sacB GTGGACAGCACCCTCGGTTTAGAAA… GlpX protein glpX
1411_slr2095-sacB ATGTCTACACACTGTCACTGTCCTC… transposase
1412_slr2096-sacB GTGGCATTTTCTTCAGGCAAAAAAA… transposase
1413_slr2097-sacB ATGTCAACTTTGTATGAAAAATTAG… cyanoglobin glbN
1414_slr2098-sacB ATGTTTTGGCAACCTGTTAGTCCCC… hybrid sensory kinase hik21
1415_slr2099-sacB ATGGATACAGCAACAACCAAGGGCA… hybrid sensory kinase
1416_slr2100-sacB ATGAACACTCCCCCCGATCCCAACT… regulatory components of sensory transduction system rre20
1417_slr2101-sacB ATGAAATTTATCTCCAGCTTCTTTG… cupin domain-containing protein
1418_slr2102-sacB ATGTTTAATTGGTTCCGTCGTCAGT… cell division protein; FtsY ftsY
1419_slr2103-sacB GTGCTAAGAGCGACCAGTGATCGAT… acyltransferase family protein
1420_slr2104-sacB ATGAAGTCTTTTTTCCTCAACACAT… hybrid sensory kinase rre22
1421_sll1973-sacB ATGACCACCGCTCTTTTACTCTGCC… glycerol-3-phosphate 1-O-acyltransferase plsY
1422_slr2105-sacB ATGGCTCGGCTTACTGCTCTATTTC… hypothetical protein
1423_sll1430-sacB ATGGATTTAAAAGCTTTAATTCGGG… adenine phosphoribosyltransferase apt
1424_sll1429-sacB ATGATATTAGTTCTTCCTTGGTTAA… restriction endonuclease
1425_ssl2789-sacB GTGGGGCAGTTTGAAAGAGAGATGA… resolvase; recombinase family protein
1426_sll1428-sacB ATGGAGTCCAATTTTTTAACAACTA… bile acid:sodium symporter family protein
1427_slr1503-sacB ATGACAATTAGCACCACAGATACTT… aldo/keto reductase
1428_sll1427-sacB ATGGCAATCCATCTGAAGGCATCAC… protease; HhoB hhoB
1429_sll1426-sacB GTGATGAAATCTTTACTGCGGGGAA… hypothetical protein
1430_sll1425-sacB GTGTGGGATAATGGGTCAGTGCGTC… proline-tRNA ligase proS
1431_slr1505-sacB ATGGATTTTCTCGCCTCCGAGTCCT… hypothetical protein
1432_sll1424-sacB ATGACCAGTGCCTATATTCTGGTTC… DUF3084 domain-containing protein
1433_sll1423-sacB ATGGATCAGTCCCTAACCCAAGATC… global nitrogen regulator ntcA
1434_ssl2781-sacB ATGCAAAAAGCCGACGAATTTGCCA… hypothetical protein
1435_slr1506-sacB ATGGAGAAAAAAATAACTTTACGTT… alpha/beta hydrolase
1436_slr1507-sacB ATGGCAGGGTTTTCCCGTGTCATAC… adenosyl-hopene transferase HpnH hpnH
1437_slr1508-sacB ATGCATATTGCTTGGTTAGGAAAAA… glycosyltransferase family 1 protein
1438_slr1509-sacB ATGACTATTTCCCGTACCATTTGCC… Na+ -ATPase subunit J ntpJ/ktrB
1439_slr1510-sacB ATGGCTGTAACGCGGGCGAAAATTG… PlsX protein plsX
1440_slr1511-sacB ATGCGCCAACGCTACATTTGTTCGG… beta-ketoacyl-acyl carrier protein synthase III fabH
1441_sll1418-sacB ATGTTGAAAAAGTCCCTATCCACTG… photosystem II oxygen evolving complex protein PsbP plsX
1442_slr1512-sacB ATGGATTTTTTGTCCAATTTCTTGA… sodium-dependent bicarbonate transport family permease
1443_slr1513-sacB ATGGCTAAACCAGCGAACAAGCTCG… hypothetical protein
1444_sll1415-sacB GTGGAACTGAAACAGGTGATCATTG… NAD(+) kinase
1445_sll1414-sacB GTGACTAAAATTCGCACTGTTTCTG… photosystem II biogenesis protein Psp29 psp29
1446_ssr2549-sacB ATGCCAGACTTCCAGGCATTTATCG… hypothetical protein
1447_slr1515-sacB ATGGTGTCTCCCATCTCTATCTGGC… putative bicarbonate transporter%2C IctB family ictB
1448_ssr2551-sacB ATGGCGATCGGAGAGCAAACCCATC… Cyanobacterial Signature Protein
1449_slr1516-sacB ATGGCTTACGCACTACCTAACTTAC… superoxide dismutase sodB
1450_slr1517-sacB ATGTCCCAGACCTATAACGTAACCC… 3-isopropylmalate dehydrogenase leuB
1451_ssr2554-sacB ATGGTTTTTATTCCTGATGAGAGCG… hypothetical protein
1452_slr1518-sacB ATGACCGAATCTTCGCCCCTAGCAC… 14-dihydroxy-2-naphtoic acid prenyltransferase menA
1453_slr1519-sacB ATGGCTGGGCTGGCCTGGGCTAAGG… pentapeptide repeat-containing protein hglK
1454_slr1520-sacB ATGCGGGGCTTAGGCAATCGCCAGC… aldo/keto reductase
1455_slr1521-sacB GTGGAATTAGCCCAGCGTTTGGGGG… GTP-binding protein; HflX hflX
1456_slr1522-sacB GTGAGAAGCTTTGAATACTTGCATC… transposase
1457_slr1523-sacB ATGACAGCTTACTTGCGAGAGCAAG… transposase
1458_slr1524-sacB ATGGCTTACAGTTTAGATTTAAGGC… transposase
1459_sll1411-sacB ATGTCTAAATCTCCCAATCGGGAAT… DUF86 domain-containing protein
1460_ssl2749-sacB ATGAAATGCCAATCAGTCCTGCAAT… DNA polymerase subunit beta
1461_sll2001-sacB GTGGCAAAATGGGGGACGGACAGTC… leucine aminopeptidase lap
1462_ssr3570-sacB ATGTCGGACGTGATGATCCGGGTAG… hypothetical protein
1463_ssr3571-sacB ATGGAAACCATTAATTATCAACAAT… type II toxin-antitoxin system Phd/YefM family antitoxin
1464_slr2107-sacB GTGAATGATAATCACTCAACACGTT… polysialic acid transport protein; KpsM kpsM
1465_slr2108-sacB ATGGGAGTGGATGGGATGACCCAGG… polysialic acid transport ATP-binding protein; KpsT kpsT
1466_sll1999-sacB ATGGATAGAGGATTTTGTGACTTAC… transposase
1467_sll1998-sacB ATGGCTTACAGTTTAGATTTGAGGC… transposase
1468_sll1997-sacB ATGATATCAAATTTTGGACATATTG… transposase
1469_slr2110-sacB ATGACTGACTATCAAGGTTATTTAG… sugar phosphate isomerase/epimerase
1470_slr2111-sacB ATGATAGTTAACCACGATAAACAAA… CBS domain-containing protein
1471_slr2112-sacB ATGGCTTACAGTTTAGATTTAAGGC… transposase
1472_slr2113-sacB ATGTTAAGAGAACTAATTAAGCTCT… transposase
1473_slr2114-sacB ATGAAATTATCCGTTCTTACTGTGG… spore coat polysaccharide biosynthesis protein; SpsC spsC
1474_slr2115-sacB ATGTTCATGCAGAAAATACAAGCCA… beta-3-deoxy-D-manno-oct-2-ulosonic acid transferase
1475_slr2116-sacB ATGAACGAAGAAAAACAACAAGAAT… spore coat polysaccharide biosynthesis protein; SpsA spsA
1476_slr2117-sacB ATGCTAATCACAAAACAATTATTCT… DUF115 domain-containing protein
1477_slr2118-sacB GTGGACATGAAATATCCTAGGATTT… glycosyltransferase family 1 protein
1478_slr2119-sacB ATGAGTTTTTCTGAACATCATCTTG… FkbM family methyltransferase
1479_slr2120-sacB ATGCCCCTTGTCTCCCTAATTATTC… glycosyltransferase
1480_slr2121-sacB ATGACTGCTTACCACCTAATTGATA… cyclase family protein
1481_slr2122-sacB ATGAACTCTGTAAAAATTGCGGCCC… acylneuraminate cytidylyltransferase
1482_slr2123-sacB ATGAAATGGAAAGTTTTAGTAACCT… D-isomer specific 2-hydroxyacid dehydrogenase family
1483_slr2124-sacB ATGAAACCAGTTGTTCTTATCACCG… short-chain alcohol dehydrogenase family
1484_slr2125-sacB ATGACGAAAAACACAACCGTAGAGC… DUF115 domain-containing protein
1485_slr2126-sacB ATGTTTTTCATGCAAAATACTAAGT… glycosyltransferase family 2 protein
1486_slr2127-sacB ATGGCCACCACCCCCCAAGAAGTCT… hypothetical protein
1487_slr2128-sacB ATGATTTCTAACCCTTCTGTCCCAA… DUF29 domain-containing protein
1488_sll1995-sacB ATGCTTGAAGCACCTTTGTTAAGTA… glycosyltransferase family 2 protein
1489_sll1994-sacB ATGTTTCCCACCATCCGTCCCCGTC… porphobilinogen synthase hemB
1490_slr2130-sacB ATGGCCACCACTATCCCCGTTCCCC… 3-dehydroquinate synthase aroB
1491_slr2131-sacB ATGTTCGTTGATTTTTTCATTAAGC… cation or drug efflux system protein
1492_sll1241-sacB ATGGGTATTACATCAAAAATATTTG… hypothetical protein
1493_sll1240-sacB ATGAATAATCATATTCAGGAAGATT… hypothetical protein
1494_sll1239-sacB GTGACCAATAATTTGACTTCAGACC… hypothetical protein
1495_slr1322-sacB ATGCCTCCTACTCTGCTCCTCTCCC… metalloprotease
1496_sll1237-sacB ATGAATAAGGGCTTTGTCTCCGGTG… protoporphyrinogen oxidase hemK, prmC
1497_slr1324-sacB GTGGAAGTAGAATACAGTGGAGTGT… hybrid sensory kinase
1498_ssr2194-sacB ATGTTTGCCTCCACCAACTACACCT… hypothetical protein
1499_sll1236-sacB ATGTCGTCTACGCTACCACTCGATT… hypothetical protein
1500_slr1325-sacB ATGAATGCCGTCGCCGCTCTGCCCA… (p)ppGpp 3-pyrophosphohydrolase spoT
1501_sll1234-sacB ATGGTAGCAACGCCCGTTAAACAGA… S-adenosylhomocysteine hydrolase ahcY
1502_ssr2201-sacB ATGCACTCATTGTCATCACTGGAAT… hypothetical protein
1503_slr1327-sacB ATGAACAATGAAGTTATCCGGGTTG… putative toxin-antitoxin system toxin component%2C PIN family
1504_sll1233-sacB ATGGCAACCTATCGCATTGAACATC… transglutaminase family protein
1505_sll1232-sacB ATGCTGCAAATTGCACCCCCATCGA… Uma2 family endonuclease
1506_sll1231-sacB GTGATGCTAAAAGTTTGTGTGGATG… Mannosyltransferase B mtfB
1507_sll1229-sacB ATGGGCCCGGACTGTAAAACCCTTT… hybrid sensory kinase hik41
1508_sll1228-sacB GTGCGTTTTGCCATAGAGCAACTGG… hybrid sensory kinase hik4
1509_slr1329-sacB ATGGTAGCCGTAAAAGAAGCAACTA… ATP synthase b subunit atpB
1510_slr1330-sacB ATGACCATTGCCTGTTATCCCCCTT… ATP synthase e subunit atpE
1511_slr1331-sacB ATGTCCGATCGCCTTGTTTGCCCTA… processing protease ymxG
1512_slr1332-sacB ATGGACCGCCAGGGGAAACAAAAAA… beta ketoacyl-acyl carrier protein synthase fabF
1513_sll1226-sacB ATGTCTAAAACCATTGTTATCGATC… hydrogenase large subunit hoxH
1514_sll1225-sacB ATGACCAACCAAACTTCTTTCACAA… PIN domain-containing protein
1515_ssl2420-sacB ATGAATACCCAATTAGTAGAATCCT… hypothetical protein
1516_sll1224-sacB ATGGCTAAAATTCGTTTTGCTACCG… hydrogenase small subunit hoxY
1517_sll1223-sacB ATGTCTGTTGTTACTTTAACCATTG… hydrogenase subunit hoxU
1518_sll1222-sacB ATGAATGTTTTAACTGCTCCCATCA… Uma2 family endonuclease
1519_sll1221-sacB ATGGACATTAAAGAATTAAAGGAAA… hydrogenase subunit hoxF
1520_sll1220-sacB ATGACCGTTGCCACCGATCGCCAAA… bidirectional hydrogenase complex protein HoxE hoxE
1521_ssr2227-sacB ATGCCATCTTTTATTTACAGCGCAC… transposase
1522_slr1334-sacB ATGGTTTACACTCCTGCTCCGATTA… phosphoglucosamine mutase
1523_sll1219-sacB ATGCACTACGCCATCCCCAAAACCT… hypothetical protein
1524_sll1218-sacB ATGAAAGTTTTAGTTATCGGCGCCA… SDR family oxidoreductase ycf39
1525_sll1217-sacB ATGTCAGAGCTTCAAACCCTGATCC… hypothetical protein
1526_ssr1375-sacB GTGTCAGCTACCCAGGTAGACGTTT… hypothetical protein
1527_slr0804-sacB ATGGTTCGCGGTTCTGAACTTTATT… D-alanyl-D-alanine carboxypeptidase dacB
1528_slr0806-sacB ATGGATTGGTCAGCCATTGCGGCTT… FAD-binding oxidoreductase glcD2
1529_sml0008-sacB ATGGACGGTTTGAAATCCTTTTTGT… photosystem I subunit IX psaJ
1530_sll0819-sacB ATGAAACATTTGTTGGCGTTGCTCC… photosystem I subunit III psaF
1531_slr0807-sacB ATGGCAATCATTTTAGCAATTGAAA… tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD gcp/tsaD
1532_slr0808-sacB ATGGCAGAACCCATGACAGAACAAC… ribosome maturation factor RimM rimM
1533_slr0809-sacB ATGCCCAGCCATTTGCCAAATGCTA… dTDP-glucose 4-6-dehydratase rfbB
1534_slr0810-sacB ATGCAATTAATCTCTGGCTTTGTGG… DUF4351 domain-containing protein
1535_sll0818-sacB ATGGGCATTCTTTATTTGGTGGCCA… 16S rRNA (cytidine(1402)-2'-O)-methyltransferase rsml
1536_ssl1520-sacB ATGGCCATGGATAAGGATACGAAAT… hypothetical protein
1537_sll0817-sacB ATGGCCAAAGTTCCGCCCCTGATTG… tRNA delta-2-isopentenylpyrophosphate (IPP) transferase miaA
1538_sll0816-sacB ATGACTAAATCCCCAAACCTAACCG… oxidoreductase
1539_sll0815-sacB ATGGCACGACAAGCTCGCTATCAAA… slipin family protein
1540_sll0814-sacB ATGGAAAAACTAATGCGGATTGAGC… carotenoid biosynthesis protein
1541_sll0813-sacB ATGAGTCGCAAAAATCTCATCCTAT… cytochrome c oxidase subunit II ctaC
1542_slr0812-sacB GTGGAAACATCAAAGCCGGGTTGTG… type III pantothenate kinase
1543_slr0813-sacB GTGAATCTTGGCCAATGGGGGGAAA… ceramide glucosyltransferase
1544_sll0812-sacB ATGGGTTCCCCAGAATTTTCTCCTA… hypothetical protein
1545_ssr1386-sacB ATGGAAGATTTATTAGGTTTGCTAC… NADH dehydrogenase subunit; NdhL ndhL
1546_slr0815-sacB GTGTTTTTTTGTTGCCCATTGTTCC… DUF3007 family protein
1547_slr0816-sacB ATGGTTATACAGCCCATTTACTTAC… DUF177 domain-containing protein
1548_sll0811-sacB GTGCAACTGTTCCGAGTTACGGTGT… IS4 transposase
1549_sll0810-sacB GTGAGCCATAACCGAATTAAACGTT… IS701 family transposase
1550_sll0809-sacB ATGGCCTTGGATCAATGGTTATTGG… lipoate--protein ligase family protein
1551_slr0817-sacB ATGTCCGTTGCGACACCCCTCCTCG… isochorismate synthase entC
1552_slr0818-sacB ATGGGGGAAACTGTCATGCGTCTCA… hypothetical protein
1553_slr0819-sacB ATGGGTTTGGCAACGGCTCCTTTAG… apolipoprotein N-acyltransferase int
1554_ssr1391-sacB GTGAAAAATACTGAAAACATAATGG… glutaredoxin family protein
1555_slr0820-sacB ATGAATAAGTGGTTTAGTAAAAAGT… glucosyltransferase
1556_ssl1507-sacB ATGAATAACGCCCCCATTCATCGAA… transposase
1557_sll0808-sacB ATGGCTTACGATCTTGATTTACGTC… transposase
1558_slr0821-sacB GTGGTTTCACCGGCTTTGCCCCGGT… MoaD/ThiS family protein
1559_sll0807-sacB ATGTCCAAAAATATCGTCGTTGCTC… pentose-5-phosphate-3-epimerase cfxE
1560_slr0822-sacB ATGTCTGACCCTGATGTGTCCGTCT… cation-transporting ATPase; E1-E2 ATPase
1561_slr0823-sacB ATGCCCCGCACCCAACGTAACGATA… photosystem I assembly protein Ycf3 ycf3
1562_sll0804-sacB ATGAATTCCCCTGCCCCTGATCCGG… DUF697 domain-containing protein
1563_sll0803-sacB ATGAATACCCCCTCTTCGACCCAAC… DUF29 domain-containing protein
1564_sll0802-sacB ATGGGGCAAAAAACTACAACTTTGT… DUF29 domain-containing protein
1565_ssl1498-sacB ATGAACAACTATACTAAAGACGATG… ssl1498 family light-harvesting-like protein
1566_sll1263-sacB ATGACCGCCCGTCTTGCCCGTCCCT… transporter
1567_sll1262-sacB ATGTTGCCATTGCCATTAATTGCTA… NAD(P)H-quinone oxidoreductase subunit N
1568_sll1261-sacB ATGGCAGAAATTACCGCTCAACTCG… elongation factor TS tsf
1569_sll1260-sacB ATGCCCGTAGTTTCTCTCGCAGATT… 30S ribosomal protein S2 rps2
1570_slr1336-sacB ATGTCCACCAAAAGTAAGATTTTTC… H+/Ca2+ exchanger
1571_sll1258-sacB ATGATTAAGAATGATATTTGGATCC… dCTP deaminase
1572_sll1257-sacB ATGTTAAGAGAACTAATTAAGCTCT… transposase
1573_sll1256-sacB ATGGCTTACAGTTTAGATTTAAGGC… transposase
1574_slr1338-sacB ATGTCAAACCAACGGGATTACACGC… VWA domain-containing protein
1575_slr1339-sacB ATGCTATTGAGGATTGAAACCAGAT… hypothetical protein
1576_slr1340-sacB ATGATTGACATTGTAAAAGCAGATC… GNAT family N-acetyltransferase
1577_sll1255-sacB ATGATATCAAATTTTGGACATATTG… transposase
1578_sll1254-sacB ATGCTGGAAATTGTAGCGGCCATTT… hemolysin
1579_slr1342-sacB ATGGCTGACCCCACCAAAATCATTG… four-carbon acid sugar kinase family protein
1580_slr1343-sacB ATGACCATTGGCATTTGGATATTGG… cryptochrome/photolyase family protein
1581_sll1253-sacB ATGGATTTAATTCTTTGCCATCAAA… polyA polymerase pcnB
1582_sll1252-sacB ATGTTACCAAGAGAAGCTTTGTTAA… photosystem II S4 domain protein
1583_slr1344-sacB ATGCGCCTATGGTTTCGTCCCCCCT… ABC transporter permease
1584_sll1251-sacB ATGCCCTCCCACCCATCCCGCAGTA… Uma2 family endonuclease
1585_ssl2471-sacB ATGGGTTGGTTGATTAATCCGAAAG… Endonuclease, Uma2 family
1586_sll1250-sacB GTGGAGTGTCGAGGCGAATCTAAAA… Endonuclease, Uma2 family
1587_sll1249-sacB ATGGTGCAGGTTTTTCGCACAATCG… pantothenate synthetase/cytidylate kinase panC/kcy
1588_slr1347-sacB ATGGCCGAAGTTTCATTGATATCCC… carbonic anhydrase icfA
1589_slr1348-sacB GTGCTGAATTCACTAATTGCTGACT… serine acetyltransferase cysE
1590_slr1349-sacB ATGAATAACCAACAACTTTGGCAAC… glucose-6-phosphate isomerase pgi
1591_slr1350-sacB ATGACTGCCACGATTCCCCCGTTGA… fatty acid desaturase desA
1592_sll1247-sacB GTGTCTTTGACCTTATCTCATGATT… hypothetical protein
1593_slr1351-sacB ATGAACTCCAAACTTTCCCTATTTG… UDP-N-acetylmuramoylalanyl-D-glutamyl-2 6-diamino-pimelate-D-alanyl-D-alanine ligase murF
1594_sll1245-sacB ATGGCCCCTGTAATCGAAAAAAGTC… cytochrome; CytM cytM
1595_sll1244-sacB ATGGAGGTTTGTGAATTGCTCCACA… 50S ribosomal protein L9 rpl9
1596_slr1353-sacB ATGCCAATGTCCAGTCTGATTTCCC… hypothetical protein
1597_sll1242-sacB ATGCGCGCCCTATTAATCTATCCGT… B12-binding domain-containing radical SAM protein
1598_slr1956-sacB ATGGTTAACCATGGTTATCCCCTAG… tetratricopeptide repeat protein
1599_slr1957-sacB ATGAGACATTTTTGCGACGCTTGGA… hypothetical protein
1600_slr1958-sacB ATGGCTGATTCCATCACCCCAGACT… GUN4 domain-containing protein