Plasmids and Cultures

This database contains entries for 3,572 known and predicted gene coding sequences in Synechocystis sp PCC 6803 based on the Kazusa genome sequence and nomenclature (http://genome.microbedb.jp/cyanobase/GCA_000009725.1). Plasmids for generating marked mutants and mutant strains can be searched for by gene Name/Accession (e.g. 'sll1226' or '1513_sll1226-sacB'),  gene homology (e.g. 'hydrogenase large subunit'), Gene ID (e.g. hoxH), or by a portion of the gene coding sequence Sequence (CDS).

Please note, the library is still being completed. Plasmids and/or marked mutants may not be available. Users can also search for available materials by Plasmids made or Mutant made.

Plasmids, mutants and the unmarking spacer plasmid pUC19K-UL are currently available on request. Regardless of the segregation status of the mutant, open reception of a cyanobacterial mutant strain we recommend 1-2 cycles of restreaking on a selection plate containing kanamycin (100 μg/ml).

Name Sort ascending CDS Gene Homology Gene ID
0972_sll1358-sacB ATGGTCAACTCGGTAATAGGATGGT… Oxalate decarboxylase odc
0971_sll1359-sacB ATGAAAATAGGAAGAATTACGGCCT… Formate dehydrogenase, c-type cytochrome
0970_slr1440-sacB ATGACAGGCCCCGTTATTTTTCACC… glyoxalase
0969_sll1360-sacB ATGGCCTACGAACCTCTGCACCATA… DNA polymerase III subunit dnaX
0968_ssr2406-sacB ATGAATGAACCAAAACAGACCGAAA… hypothetical protein
0967_sll1362-sacB GTGACTGAAGCCAAAAGTTATAAAG… isoleucyl-tRNA synthetase ileS
0966_slr1438-sacB ATGTACAACATTAGCTTTACCCCCG… aldose 1-epimerase
0965_slr1437-sacB ATGTCCGCTGAATTTTTTGACTGCC… hypothetical protein
0964_slr1436-sacB ATGGATAATGTTACTCGCTATCAAA… class I SAM-dependent methyltransferase
0963_slr1435-sacB ATGACCACCGATCTATCGGTTTACC… PmbA protein pmbA
0962_slr1434-sacB ATGTCTAACAGTTTACAAACCGTGG… pyridine nucleotide transhydrogenase beta subunit pntB
0961_ssr2067-sacB GTGATTATGACTGTTAGTAATATTT… type II toxin-antitoxin system HicB family antitoxin
0960_slr1241-sacB ATGCAAATTTCCCCAGCATTTAAAT… hypothetical protein
0959_slr1240-sacB ATGGCCAGAAAATCAAAAGAATTTC… hypothetical protein
0958_slr1239-sacB ATGACCATTGCCGCCCCTAAAACCA… pyridine nucleotide transhydrogenase alpha subunit pntA
0957_sll1127-sacB ATGGATTGGCACATTGCAAAACATT… 1%2C4-dihydroxy-2-naphthoyl-CoA synthase menB
0956_ssr2062-sacB ATGAAAACCGCTAACTTTATCACCT… hypothetical protein
0955_slr1238-sacB GTGAAACTGGCTTTTATTATCGATC… glutathione synthetase gshB
0954_ssr2061-sacB ATGGCTGTCTCGGCAAAAATTGAAA… glutaredoxin 3 grxC/grx2
0953_ssr2060-sacB GTGGTAGGATTAGGCGAAAATAATC… 2-hydroxy-6-oxohepta-24-dienoate hydrolase
0952_sll1129-sacB ATGACTCCTTCCCTAACCCTTCCCG… 2-hydroxy-6-oxohepta-24-dienoate hydrolase todF
0951_ssl2245-sacB ATGTCTATCAATGCTTACAAACTAG… hypothetical protein
0950_sll1130-sacB ATGAATACAATTTACGAACAATTTG… type II toxin-antitoxin system PemK/MazF family toxin
0949_slr1237-sacB ATGTCTTCCCCTCTGGTTCTTCCCA… cytosine deaminase codA
0948_sll1131-sacB ATGATTACCAGGGAAACCTTGCCCG… GtrA family protein
0947_sll1132-sacB ATGATGGGTAAATTTTTTCACTTCA… hypothetical protein
0946_ssl2250-sacB ATGTATATCTGCGTTTGCCGGGGTA… bacterioferritin-associated ferredoxin glycoprotein 64
0945_slr1236-sacB ATGACCCCATTGACTTTTCGTAACT… DUF192 domain-containing protein
0944_slr1235-sacB ATGCAGTCCTTTTCGCCCCAGATCA… alpha/beta fold hydrolase
0943_slr1234-sacB ATGGCAGAAGATACCATTTTCAGTA… protein kinase C inhibitor
0942_ssr2049-sacB ATGCTTTGGCACGTATTTTACGGTC… protochlorophillide reductase 57 kD subunit bchB
0941_slr1233-sacB ATGCTCGAACAGGACGTTGTCATTG… succinate dehydrogenase flavoprotein subunit frdA
0940_ssr2047-sacB ATGTCCATAATGTTGCAAGACCCCC… hypothetical protein
0939_slr1232-sacB ATGAAAGGTCACATCAATGCTCCGG… SH3 domain-containing protein
0938_sll1135-sacB ATGAAGCAAATATCCGTGGTTATTG… NAD(P)/FAD-dependent oxidoreductase
0937_slr1230-sacB GTGAAAAATATTTTGCTTTGCACTG… universal stress protein
0936_slr1229-sacB ATGATCAATTGGACGATATTAAAGC… SulP family inorganic anion transporter
0935_slr1228-sacB ATGCAAACTTCCCCATCTTCCACTG… peptide-chain-release factor 3 prfC
0934_slr1227-sacB GTGTCAAACCAGAACAAAAGCGTGA… chloroplast import-associated channel IAP75 IAP75
0933_slr1226-sacB GTGCAGATGTGGCCCCCAGAGCCCT… phosphoribosyl aminoidazole succinocarboxamide synthetase purC
0932_sll1138-sacB ATGTTAAAGGCAGTATTGTTAGACT… HAD family phosphatase
0931_slr1225-sacB ATGGATCTGCTCTGCACCCGCCCCG… protein kinase; PknA pknA
0930_slr1224-sacB GTGGCCCAGGTAATTGTCGAAAATC… cytoplasmic membrane protein for maltose uptake malK
0929_slr1223-sacB ATGGCCAGTTGGTCACTAAGGACCG… cell division inhibitor sulA
0928_sll1142-sacB ATGATTGAGATGAGAGTAGCTGGCA… bifunctional nuclease family protein
0927_sll1143-sacB GTGCCCATGACTGTTGTCCCCGACT… DNA helicase II uvrD
0926_slr1222-sacB ATGATTGGACAGGTAGTTAGGCTTG… hypothetical protein
0925_slr1220-sacB ATGACCATTGATAACATCCGTCTTA… photosystem reaction center subunit H
0924_slr1219-sacB ATGGTGATTTTTGATCATCGCCTAG… urease accessory protein E ureE
0923_slr1218-sacB ATGGTTATTAGCTCACGTCCCTTAA… DUF4168 domain-containing protein
0922_sll1144-sacB ATGGGAGCCAAATCCATGGAAGGAA… 16S rRNA (cytosine(1402)-N(4))-methyltransferase rsmH
0921_slr1752-sacB ATGGTTCAAACTGCCCTACTTCCCC… Uma2 family endonuclease
0920_slr1751-sacB ATGTTGAAACAAAAACGCAGTCTGA… carboxyl-terminal protease prc
0919_sll1632-sacB ATGACGGATTTAGTTGTGTCTGATT… cell division protein FtsQ/DivIB ftsQ/divlB
0918_sll1633-sacB ATGACGCTCAATAATGATTTACCCC… cell division FtsZ protein ftsZ
0917_ssl3177-sacB GTGATGGCCCAATCCGCCACTTTCT… rare lipoprotein A repA
0916_slr1748-sacB ATGGCCCTCAAACTTTATTTCCTGC… histidine phosphatase family protein
0915_sll1634-sacB ATGAGCAAGGAATTTCGAGAGCTAT… hypothetical protein
0914_sll1450-sacB ATGAGTAATTTTTCCCGAAGCACCC… nitrate ABC transporter substrate-binding protein nrtA
0913_sll1451-sacB ATGGCTAGTTCCACCGCTGGGCTCC… nitrate ABC transporter%2C permease protein nrtB
0912_sll1452-sacB ATGATGCCCTTCATTGAAATTGATC… ATP-binding cassette domain-containing protein nrtC
0911_sll1453-sacB ATGCAAACAATGAATGTCAATGACC… ATP-binding cassette domain-containing protein nrtD
0910_sll1454-sacB ATGGATTCACCAGCTATTCTTCCCA… nitrate reductase narB
0909_sll1455-sacB ATGTTTTTTCAATTTGAAGCTGATT… hypothetical protein
0908_slr1552-sacB ATGTGGCGTTATCGGTCACCCGAAA… hypothetical protein
0907_sll1456-sacB ATGCAAAAATTTTTTAAACAATTAC… FkbM family methyltransferase
0906_sll1457-sacB GTGTCAACCTCTTTTTCCAGTGCCT… glycosyltransferase rfbJ
0905_slr1550-sacB ATGGCCGATTCCCATTCCCACTCCA… lysyl-tRNA synthetase lysS
0904_ssr2611-sacB GTGACCCCTAAAAGTGGTATTCTTC… hypothetical protein
0903_slr1549-sacB ATGACTGCCACCATCCTCGTTGAAA… polypeptide deformylase def
0902_sll1459-sacB ATGAATTTTTTGCTCACCAATGACG… 5'/3'-nucleotidase SurE surE
0901_sll1461-sacB ATGGTTATCGCCCCCCATCGCACTA… Uma2 family endonuclease
0900_sll1462-sacB GTGAACTTAGTCTGTCCCGTTCCCC… hydrogenase expression/formation protein; HypE hypE
0899_sll1463-sacB ATGGCCATCAAACCCCAACCCCAAT… cell division protein; FtsH ftsH
0898_sll1464-sacB GTGACTAATCCCTTTCTGAATTTGA… YdiU family protein
0897_ssl2823-sacB GTGACCCTCCAGGAGCAAAAAATGT… twin-arginine translocase TatA/TatE family subunit tatA
0896_sll1466-sacB ATGAACGTTCTCTTTCTTCACCAAA… glycosyltransferase
0895_slr1547-sacB ATGATTAATTTGGTTGAATTTAATC… permease
0894_slr1546-sacB GTGTCCAACTTTGTGAGGAGTGAAT… anti-sigma factor
0893_slr1545-sacB GTGTTGGGTAAATCCATGAATCAAG… RNA polymerase sigma-E factor sigG
0892_slr1544-sacB ATGAACTACCAAAGGACTGCCCTTG… member of the SCP family lilA
0891_ssr2595-sacB ATGACTAGCCGCGGATTTCGCCTCG… high light inducible protein hilB/scpD
0890_sll1468-sacB GTGTGCCAGGAGTCCGTCATAGTAA… b-carotene hydroxylase bhy
0889_slr1543-sacB GTGAATTTTGACCGCATTTTTGCTT… DNA-damage-inducible protein: MATE family efflux transporter
0888_slr1542-sacB ATGACTGCTCTACGCATCGGCAACG… 2-C-methyl-D-erythritol 2%2C4-cyclodiphosphate synthase
0887_slr1541-sacB ATGGCCACCACTGCCGATGAAGTTT… DUF3782 domain-containing protein
0886_slr1540-sacB ATGCGCATTTTAATTATGGGAGGAA… NAD-dependent epimerase/dehydratase family protein
0885_slr1538-sacB GTGTTTGCCTGTGCGTCGGCGATCG… cobalamin biosynthesis protein CbiD cbiD
0884_slr1537-sacB ATGGTTAATCAGCCCCTAGTAAGCA… glycosyltransferase family 2 protein
0883_slr1536-sacB ATGGCTGATCGCCAATCGTTGGAAG… ATP-dependent DNA helicase; RecQ recQ
0882_slr1535-sacB ATGGTATCGATCGCCGAACGGACAA… hypothetical protein
0881_sll1469-sacB GTGATGACTGAGCAGAAATACTTGA… GNAT family N-acetyltransferase
0880_sll1712-sacB GTGTCTATCCGGGCTGTGGAGCCTC… DNA binding protein HU
0879_sll1713-sacB ATGGCAGGCTGTCCAGCTTTTTCTT… histidinol-phosphate aminotransferase hisC
0878_sll1714-sacB ATGCTCAGAGAATTAAGAACAAACA… hypothetical protein
0877_sll1715-sacB GTGAACCTGATTGGTCAGAGAAAAT… PIN domain-containing protein
0876_sll1716-sacB ATGATAATCCGGGAATTTTACAGCA… transposase
0875_sll1717-sacB ATGAGCGTTCTTAAATGGATAAATG… hypothetical protein
0874_slr1844-sacB ATGCCCGAACAAAATAGCATCCGTA… excinuclease ABC subunit A uvrA
0873_slr1843-codA ATGGTAACGCTACTCGAAAATCCCT… glucose 6-phosphate dehydrogenase zwf
0872_slr1842-sacB GTGGGGTTTTTCGTTGTAAGATGCA… cysteine synthase cysK
0871_slr1841-sacB ATGCTTAAACTATCTTGGAAGTCCC… membrane protein
0870_slr1840-sacB ATGCGTCAAATTTTGATTGCACCAG… glycerate kinase
0869_slr1839-sacB ATGTCCGCCCAATCCGCCGTCGGTT… carbon dioxide concentrating mechanism protein; CcmK ccmK
0868_slr1838-sacB ATGCCCCAGGCCGTTGGCGTGATAC… carbon dioxide concentrating mechanism protein; CcmK ccmK
0867_sll1721-sacB ATGGCTGAGACCCTACTGTTTGCCG… pyruvate dehydrogenase E1 beta subunit pdhB
0866_slr1837-sacB ATGGCCAATATCCTCTTAGTGGACG… response regulator transcription factor
0865_sll1722-sacB ATGCACTCCATTCAATCACCCCGAC… glycosyl transferase
0864_sll1723-sacB ATGGCCATCGCCTACGTTGTTAAAC… colanic acid biosynthesis glycosyltransferase WcaL wcaL
0863_sll1724-sacB ATGAAGATTGCTTACATCTGTGTCG… LPS glycosyltransferase; IcsA icsA
0862_sll1725-sacB ATGAACCGTGCCTCCCCCATGACCC… ABC transporter
0861_sll1726-sacB ATGATGGCCGAAATTTTCTCTGATC… phosphotransferase
0860_slr1835-sacB ATGGCAACTAAATTTCCTAAATTTA… P700 apoprotein subunit Ib psaB
0859_slr1834-sacB ATGACAATTAGTCCACCCGAAAGAG… P700 apoprotein subunit Ia psaA
0858_sll1730-sacB ATGATCTCCATAGATAAAAGTTATG… hypothetical protein
0857_sll1732-sacB ATGTTAGAAAGTTTAAGTCGAATTA… NADH dehydrogenase subunit 5 ndhF
0856_sll1733-sacB ATGCTTAGCCTGCTCTTAATCCTGC… NADH dehydrogenase subunit 4 ndhD3
0855_sll1734-sacB ATGACTACCCTGACCCCAAAAGCTA… CO2 hydration protein
0854_sll1735-sacB ATGCCTAACATTGTTGAAATTGCGG… fasciclin domain-containing protein
0853_sll1736-sacB GTGAACAACTTTTCCCCCAAATTTG… hypothetical protein
0852_slr1830-sacB ATGTTTTTACTATTTTTTATCGTTC… class III poly(R)-hydroxyalkanoic acid synthase subunit PhaE phbC
0851_slr1829-sacB ATGGAATCGACAAATAAAACCTGGA… class III poly(R)-hydroxyalkanoic acid synthase subunit PhaE phaE
0850_slr1828-codA ATGGTTAACACCTACACCGCCGAAA… ferredoxin petF
0849_sll1737-sacB ATGGCATCTAACAGTACGGCAGACG… Tic20 family protein
0848_sll1738-sacB ATGTCTGATAGTCGTTTAAATCTGG… hypothetical protein
0847_sll1739-sacB ATGAAATTTAACACGGAATGCTTAC… hypothetical protein
0846_slr1827-sacB ATGTCGTTTTTTGCTCAACACACCA… alpha/beta fold hydrolase
0845_slr1826-sacB ATGCAGTTTTGCTGGATCATAATTG… hypothetical protein
0844_sll1740-sacB ATGACCATGAACGCCCAAGCCATAA… 50S ribosomal protein L19 rpl19
0843_ssl3335-sacB GTGGTTAAGAAGGAAGCGGTCAGAA… secretory protein; SecE secE
0842_sll1742-sacB ATGAGCTTCACTGACGATCAATCTC… transcription antitermination protein; NusG nusG
0841_sll1743-sacB ATGGCCAAGAAAGTCGTCGCTCTGA… 50S ribosomal protein L11 rpl11
0840_sll1744-sacB ATGACTAAAAAGCTTTCAAAACGCA… 50S ribosomal protein L1 rpl1
0839_sll1745-sacB ATGGGAAGAACGAGAGAAAATAAAG… 50S ribosomal protein L10 rpl10
0838_sll1746-sacB ATGTCCGCTGCAACCGATCAAATTT… 50S ribosomal protein L12 rpl12
0837_sll1747-sacB ATGGGAAACACGTTTGGCAGTTTAT… chorismate synthase aroC
0836_ssl3712-sacB GTGGGGGAAACCAAATTGACAGCAG… hypothetical protein
0835_slr2078-sacB ATGTGGCATCTTCCTCCCAGTAATT… NUDIX hydrolase
0834_slr2077-sacB ATGAATGGTATTCACCGTTGGCTTT… EAL domain-containing protein
0833_slr2076-sacB ATGGCTAAATCCATCATTTACAATG… 60kD chaperonin 1 groEL
0832_slr2075-sacB ATGACTTCTATGGCCGCTATTTCCA… 10kD chaperonin groES
0831_sll1965-sacB GTGTGGTTTCCCGAACTCAGGGCAA… hypothetical protein
0830_slr2074-sacB ATGCATGATTCCCCTGAAGCTTCTA… mannose-6-phosphate isomerase manA
0829_slr2073-sacB ATGATTTTGACCAAGTTAAAAGACT… cell division protein SepF sepF
0828_slr2072-sacB ATGACCACCGATTACCTCGAAAGAA… L-threonine deaminase ilvA
0827_sll1967-sacB ATGACCCAAAGTAATAATTTCGCCA… RNA methyltransferase
0826_sll1968-sacB GTGTGCAGTAATTCTCAAAGCGGTC… anti-sigma regulatory factor pmgA
0825_sll1969-sacB GTGGTAGCAGAATTTCCGGACCGTC… triacylglycerol lipase
0824_slr2071-sacB ATGTTTAAATTCCTTGCCAATAATT… hypothetical protein
0823_slr2070-sacB GTGCTAAATGTCCGGAGACTCCACC… DUF1868 domain-containing protein
0822_sll1971-sacB ATGCACTCACAAAAATTGGTCACAG… glycosyltransferase family 1 protein
0821_slr1216-sacB ATGACAGAGGTCACTACGACTGTTC… Mg2+ transporter mgtE
0820_sll1112-sacB ATGACAACGGTGTGGAAAGTATTGG… carbonic 3-dehydroquinase aroQ
0819_slr1215-sacB GTGGTCTTAAACCGTTTAGCGTTGG… hypothetical protein
0818_slr1214-sacB ATGACTGCTGTGATCACCCGCCAAA… PatA subfamily rre15/lsiR
0817_slr1213-sacB ATGGTAACCAAAATACTGATTGTAG… AraC subfamily rre14/uirR
0816_slr1212-sacB ATGGCAATCACCGCATTTACCCTGG… ethylene response sensor protein etr1/hik44/uirS
0815_slr1211-sacB ATGCCTCAATTTAAAGCTATTACGA… CobN protein cobN
0814_slr1210-sacB ATGATTAAATTCTATAGCAACGAAA… hypothetical protein
0813_slr1209-sacB ATGAATAGGAGACTGGTAGAGTCTT… DUF433 domain-containing protein
0812_slr1208-sacB ATGAAGAGTTTATGGGATGACCAGG… short-chain alcohol dehydrogenase family
0811_ssr2016-sacB ATGTTCGCCCCCATCGTTATCTTGG… hypothetical protein
0810_slr1207-sacB GTGCCCATGGCCATGCCCCTTGTCC… HlyD family efflux transporter periplasmic adaptor subunit
0809_slr1206-sacB ATGGAAATGTTTGCCCCCGGTCAAG… DUF1997 domain-containing protein
0808_slr1205-sacB ATGTTGGATCAGGGCCCGTTGGCAA… ferredoxin component, Rieske (2Fe-2S) protein
0807_slr1204-sacB ATGTCAGCCCAAGCCGTTTTCCCAA… serine protease; HtrA htrA
0806_slr1203-sacB ATGACAACACTGTATGAAACGGACT… DUF29 domain-containing protein
0805_slr1202-sacB ATGACTGCTTCTGGGTCCGATCGCC… lactose transport system permease protein; LacF lacF
0804_slr1201-sacB ATGCAAAATTCTGCCCCCCCTTCTG… urea ABC transporter permease subunit UrtC urtC
0803_slr1200-sacB GTGTCAGTACTCCTAGAAGGCATTT… high-affinity branched-chain amino acid transport protein livH
0802_slr1199-sacB ATGGGCATAGCAGAAAAGTCGATGA… DNA mismatch repair protein; MutL mutL
0801_slr1198-sacB ATGGCCTTACAACTCGGTGATGTTG… rehydrin or peroxiredoxin
0800_ssr2009-sacB ATGCTACCACCTCTAAAAAATCCAA… DUF2442 domain-containing protein
0799_sll1118-sacB ATGCTGACTTCCACAACGGATATTG… YbjQ family protein
0798_slr1197-sacB ATGGCGAATTCTGAACAGGCCTATT… DNA-protecting protein DprA dprA
0797_slr1196-sacB ATGTATCCATCCTCGTCCCCGGGCC… hypothetical protein
0796_slr1195-sacB ATGGAAGTGCAATTTCGGGAAGTAA… DUF3531 family protein
0795_slr1194-sacB ATGGCCAATCGTACCCAAAGTCGTT… DUF533 domain-containing protein
0794_sll1119-sacB ATGCCCCCCCTGCAACTACTCCGAT… phosphotransferase enzyme family protein
0793_sll1120-sacB ATGGTTTATGTCAAGCGCATCGAAC… chromosome segregation protein SMC1 smc
0792_sll1121-sacB ATGGGTAGATTGAGGGGAACACTAA… ATP-dependent helicase
0791_sll1123-sacB GTGCTATTTTTGTGCAACTTGCTTT… hypothetical protein
0790_sll1124-sacB ATGACTTTTGCCGCCACTCCCAGGG… sensory transduction histidine kinase hik3
0789_ssl2233-sacB GTGGCTAATATCAAGTCCGCCCTGA… 30S ribosomal protein S20 rps20
0788_sll1786-sacB ATGCATTTAGTTGATACCCATGTCC… YchF/TatD family DNA exonuclease
0787_sll1787-sacB ATGACAAACCTTGCCACCACGATGT… RNA polymerase beta subunit rpoB
0786_sll1789-sacB ATGACGTTTTATAACTACACCATCG… RNA polymerase beta prime subunit rpoC2
0785_sll1791-sacB ATGAAAAGTCTGACAGCCTATGAAA… transposase
0784_sll1792-sacB GTGAAAGGACGAAAGCGTCATCTAA… transposase
0783_slr1903-sacB GTGGCATTTTCTCCAGGCAAAAAAA… transposase
0782_slr1902-sacB ATGTCTACACACTGTCACTGTCCTC… transposase
0781_slr1901-sacB GTGGTGGAAAATTTGAGCAAGGTTT… ABC transporter
0780_slr1900-sacB ATGGGGAGTTATAGCCAATTAGACC… Cyanobacterial Signature Protein
0779_slr1899-sacB GTGGGAGCCTACAACTATTCCGAAG… urease accessory protein F ureF
0778_slr1898-sacB ATGAGCAGTACCCAAGATTATATCG… N-acetylglutamate kinase argB
0777_slr1897-sacB ATGGTTTCATGGTGCCGATGGCGAT… sugar ABC transporter substrate-binding protein srrA
0776_sll1796-codA ATGTTTAAATTATTCAACCAAGCTA… cytochrome c553; petJ petJ
0775_slr1896-sacB ATGGCTGAAGTTATCCCCAACAATC… DUF1823 family protein
0774_slr1895-sacB ATGCCAAGCAAGGGAAGGGGAAACG… metallophosphoesterase
0773_sll1797-sacB GTGGATGTGATTGACATGGCTCCAG… DUF98 domain-containing protein ycf21