Plasmids and Cultures

This database contains entries for 3,572 known and predicted gene coding sequences in Synechocystis sp PCC 6803 based on the Kazusa genome sequence and nomenclature (http://genome.microbedb.jp/cyanobase/GCA_000009725.1). Plasmids for generating marked mutants and mutant strains can be searched for by gene Name/Accession (e.g. 'sll1226' or '1513_sll1226-sacB'),  gene homology (e.g. 'hydrogenase large subunit'), Gene ID (e.g. hoxH), or by a portion of the gene coding sequence Sequence (CDS).

Please note, the library is still being completed. Plasmids and/or marked mutants may not be available. Users can also search for available materials by Plasmids made or Mutant made.

Plasmids, mutants and the unmarking spacer plasmid pUC19K-UL are currently available on request. Regardless of the segregation status of the mutant, open reception of a cyanobacterial mutant strain we recommend 1-2 cycles of restreaking on a selection plate containing kanamycin (100 μg/ml).

Name Sort descending CDS Gene Homology Gene ID
1201_slr1739-sacB ATGATGACCCTCACTCCCACCATCG… photosystem II reaction center protein Psb28 psb28
1202_slr1740-sacB GTGTTGCTAAATCTTCCCGCCACCG… peptide ABC transporter substrate-binding protein
1203_sll1618-sacB ATGTTGCCGTTGATTTTACCCGCTC… hypothetical protein
1204_slr1742-sacB ATGGAATTAACAATCGGCTGGCTTT… hypothetical protein
1205_slr1743-sacB ATGACGGACGCTCGACCACGGATTT… NADH dehydrogenase ndhB
1206_slr1744-sacB ATGTCAAGATTGCCTGGTTTTGCTC… N-acetylmuramoyl-L-alanine amidase amiA
1207_slr1746-sacB ATGCGTGAGCCCCAGCGTAGCCGAA… glutamate racemase murI
1208_slr1747-sacB ATGACCAGTTTGTTAACCTCCTCTG… cell death suppressor protein Lls1 lls1
1209_slr0851-codA ATGAATTCCCCCACCTCTCCCCGTC… NADH dehydrogenase ndbA
1210_slr0852-sacB ATGTTACACTTCGCCCAAATTACCA… hypothetical protein
1211_slr0853-sacB ATGGTTTCGCTTCTTGGCCCCAAAT… ribosomal-protein-alanine acetyltransferase rimI
1212_slr0854-sacB ATGGGGAGGCAGTTGATTTTGTTTC… deoxyribopyrimidine photolyase phr
1213_sll0858-sacB GTGGTTACTAAGCGATCCCCCACGG… periplasmic heavy metal sensor
1214_sll0857-sacB ATGCTCGGCGGGCCATGCGGGAATT… hypothetical protein
1215_sll0856-sacB ATGATCCAGCGAAGTCAGCAGGGTG… RNA polymerase sigma-E factor sigH
1216_sll0855-sacB ATGGCTGCCGATTTTGAAACCTCTA… H(+)/Cl(-) exchange transporter ClcA clcA
1217_sll0854-sacB ATGGCATTTTTTAAAGAAGACCCGG… Cyanobacterial Signature Protein
1218_sll0853-sacB ATGAAGCCTGTTGCCCCAAGAAGCT… Chromophore lyase CpcS/CpeS 1
1219_slr0856-sacB ATGGCTTACAGTTTAGATTTGAGGC… transposase
1220_slr0857-sacB ATGTTAAGAGAACTAATCAAGCTCT… transposase
1221_sll0851-sacB GTGGTAACGCTCTCTAATACTTCGA… photosystem II CP43 protein psbC
1222_sll0849-sacB ATGACTATTGCAGTCGGACGCGCCC… photosystem II D2 protein psbD
1223_sll0848-sacB ATGGTCTCCTGCGAAAATCTTTGGC… chromosomal replication initiator protein; DnaA dnaA
1224_sll0847-sacB GTGGGGGTTAACCGGCTAGGGATCT… DUF202 domain-containing protein
1225_slr0861-sacB ATGGCCAATCCTGTCACTTTGCCCC… glycinamide ribonucleotide transformylase purT
1226_sll0846-sacB ATGAAATTTTCCCTCGAGTCTCTCT… DUF1232 domain-containing protein
1227_slr0862-sacB ATGCTGATTTCCCGTGCCCCTGTCC… LmbP protein lmbP
1228_slr0863-sacB ATGAACTTAGCCACCATTAAAGTCA… metalloprotease
1229_sll0844-sacB ATGACGCAAAAAGTAGTGGTGGGAC… tRNA 2-thiouridine(34) synthase MnmA mnmA
1230_sll0843-sacB ATGAAGCTAGCATTTTTTCAAAAAT… hypothetical protein
1231_sll0842-sacB GTGCCCCACCATTACGACAGGGTAA… neopullulanase nplT
1232_slr0864-sacB ATGCTCCGACTCGAACACATCCGCA… ABC-F family ATP-binding cassette domain-containing protein
1233_slr0865-sacB ATGGTCTCCGTTCAAAATTCCCTCC… L-histidine N(alpha)-methyltransferase egtD
1234_sll0839-sacB ATGCCAAGACCTTGCTGTTTAAAAT… hypothetical protein
1235_sll0838-sacB ATGACCCCAGACCAAGTAATTGTTG… orotidine 5 monophosphate decarboxylase pyrF
1236_sll0837-sacB ATGGAAAAATTTACTGTGTCAAAAC… tetratricopeptide repeat protein
1237_slr0867-sacB GTGCGTTTATTGTATCTAGCCCGAC… hypothetical protein
1238_slr0868-sacB GTGGAACTATCATTGTTTTACCAAG… hypothetical protein
1239_slr0869-sacB ATGTCCAAGATTGCGCCCCAATGTC… hypothetical protein
1240_slr0870-sacB ATGCAAATTATCTACGACGAATTCC… hypothetical protein
1241_slr0871-sacB ATGAAAACCGACAAAATTGGCTACC… hypothetical protein
1242_slr0872-sacB ATGTTAAGCAGTTGTGCACCGCCCG… LPS export ABC transporter periplasmic protein LptC lptC
1243_sll1165-sacB ATGGATTGCCGACTTAAGTGCACTA… DNA mismatch repair protein mutS
1244_slr1243-sacB ATGGATTTTACTAAAATAAAGAGAA… ATP-binding protein
1245_sll1164-sacB ATGAAACATGTTTTTTCTGGCTTTC… EamA family transporter
1246_sll1163-sacB GTGATTCCGTCGGTAAAACCTGTCC… cupin domain-containing protein
1247_sll1162-sacB ATGATGGATTTTCAAGATACCTGGG… DUF4079 domain-containing protein
1248_sll1161-sacB GTGAATAAAGTGCCGACTCTTATCT… adenylate cyclase
1249_sll1160-sacB ATGAATATTCGTCCCCTTGTTAGTT… hypothetical protein
1250_sll1159-sacB GTGAATTTACAAATTGAATTATACA… AhpC/TSA family protein
1251_sll1158-sacB ATGATATCTCAATCAATAATTCCCA… hypothetical protein
1252_slr1245-sacB ATGGATCGTCTTGCTTGTATGCAGA… transcriptional regulator
1253_ssr2078-sacB ATGAAAAGTCTGACAGCCTATGAAA… transposase
1254_slr1246-sacB GTGAAAGGGCGAAAGCGTCATCTAA… transposase
1255_sll1157-sacB GTGGCATTTTCTTCAGGCCAAAAAA… transposase
1256_sll1156-sacB ATGTCTACACACTGTCACTGTCCTC… transposase
1257_sll1155-sacB ATGGCGCTCAGCGGGGTCGCCATTG… Cyanobacterial Signature Protein
1258_sll1154-sacB GTGAGTTCTTTCCAGACCCTCCGTC… quinolene resistance protein; NorA; MFS transporter norA
1259_slr1247-sacB ATGCTTAGTTCACTGCAAAAAGTTG… phosphate ABC transporter substrate-binding protein PstS pstS
1260_slr1248-sacB ATGGTGGAAGGTTTTTCACGTCGTT… phosphate ABC transporter permease subunit PstC pstC
1261_slr1249-sacB ATGAAAAGTTCTGCCCCTAGTAGTT… phosphate ABC transporter permease PstA pstA
1262_slr1250-sacB ATGACTAATTTGTCCGGTTCAATGC… phosphate ABC transporter ATP-binding protein pstB
1263_slr1251-sacB ATGATGAGCAAAGTTTTTTTTGATA… peptidyl-prolyl cis-trans isomerase cyp
1264_ssl2296-sacB ATGGCCACCCCCCAACGTTTAACTG… pterin-4a-carbinolamine dehydratase dcoH
1265_sll1151-sacB ATGTCAAAGTCCAAGTCCTCCGGGC… DUF389 domain-containing protein
1266_ssr2087-sacB ATGTTTGGCGTTCTGCTGTTCCTTA… Cyanobacterial Signature Protein
1267_sll1150-sacB GTGATTATCCTAGTCAAAAATCCAC… membrane protein
1268_slr1253-sacB GTGTATTGTTCCATTGTCAGACCAG… hypothetical protein
1269_slr1254-sacB ATGCGCGTTGTGATCGCCGGAGCCG… phytoene desaturase pds
1270_slr1255-sacB ATGGCTAACGGTCAAATTTCTCCCC… phytoene synthase pys
1271_sll1147-sacB ATGACAAAAACCGAGTTACTCTGGC… glutathione S-transferase hik2
1272_slr1256-sacB ATGCAGCTAAGCCCCCAGGAAAAAG… urease gamma subunit ureA
1273_slr1257-sacB ATGTTAATCCTGCGTCGCCTAATAC… transporter substrate-binding domain-containing protein
1274_slr1258-sacB ATGAGTACCATCAAGGCCCTGTTAC… SIMPL domain-containing protein
1275_slr1259-sacB ATGCTGTTTCGTCAATTGTTTGATC… MBL fold metallo-hydrolase
1276_slr1260-sacB ATGGTCAGTTGGTGTCAGTCCCTTT… Cyanobacterial Signature Protein
1277_slr1261-sacB ATGGCTAGCTCAACCCAAACCAATG… DUF2892 domain-containing protein
1278_slr1262-sacB ATGGCACTCCAAGTGGTGGGCTATT… sulfite exporter TauE/SafE family protein
1279_slr1263-sacB ATGCTTTCCCTGCTCACACCTCTAG… DUF3370 domain-containing protein
1280_sll1961-sacB GTGATGGCTTGGGCAAGGTTACCAG… GntR family transcriptional regulator
1281_sll1960-sacB GTGCAATTAAGCTTTCAAGAACGTT… hypothetical protein
1282_sll1959-sacB ATGTTACCAGAGGTTGAACAAAGAC… extragenic suppressor; histidinol-phosphatase suhB/hisN
1283_sll1958-sacB ATGGGAGAAGCTTTAAACAAAGGGA… histidinol phosphate aminotransferase hisC
1284_sll1957-sacB GTGAAAACAACGGAAAAAGACCTAA… arsenical resistance operon repressor arsA
1285_slr2057-sacB ATGAAAAAGTACATTGCTGAATTTA… water channel protein apqZ
1286_slr2058-sacB ATGTCCAAACTCGTTATCGTTGAAT… DNA topoisomerase I topA
1287_slr2059-sacB ATGAAGAAGCGTGTGACCCTGACTT… ferredoxin
1288_slr2060-sacB ATGGAATCCCCCATCCAAGCCCAAC… 1-acyl-sn-glycerol-3-phosphate acyltransferase
1289_sll1956-sacB ATGATTCATGCTATTGAGACAACGT… Uma2 family endonuclease
1290_sll1954-sacB ATGACCAATTTCCCCCCTTTACTAA… hypothetical protein
1291_ssl3692-sacB ATGAACTCTCAGGTTAAAACTCCTT… DUF2442 domain-containing protein
1292_slr2062-sacB ATGAAGCTAAGGTATCAGTACCGAT… transposase
1293_sll1951-sacB ATGGCACTAAGTCCTAACGTAATTG… hemolysin
1294_sll1950-sacB ATGTCTTCCCCCAGCCTTTCCCCCC… FkbM family methyltransferase
1295_sll1949-sacB ATGAAGAAGCGATCTCTGTTCTCCC… hypothetical protein
1296_slr2067-sacB ATGAGTATCGTCACGAAATCAATCG… allophycocyanin a chain apcA
1297_slr1986-sacB ATGCAAGACGCAATTACGGCTGTAA… allophycocyanin b chain apcB
1298_ssr3383-sacB ATGCGGATGTTTAGAATTACGGCTT… phycobilisome LC linker polypeptide apcC
1299_sll1909-sacB ATGGCTGCGGCTAATAGTTGGTGGG… ribosomal protein L11 methyltransferase; PrmA prmA
1300_sll1908-sacB GTGAATCTAGCCTGGCTCCAGGGAC… phosphoglycerate dehydrogenase serA
1301_slr1990-sacB GTGATTCCCCTTTTAACCACCGCCT… Cyanobacterial Signature Protein
1302_slr1991-sacB GTGGATAAGCCTGCCCTACCCATTT… adenylate cyclase cyaA
1303_slr1992-sacB ATGCCATTACCCACTTCCCTGACCA… glutathione peroxidase
1304_slr1993-sacB ATGGCCGCCCATCCCAACCCATGGC… acetyl coenzyme A acetyltransferase (thiolase) thl
1305_slr1994-sacB ATGTTAAGTCTTGGTTTGGAAGATA… 3-ketoacyl-acyl carrier protein reductase fabG
1306_sll1906-sacB ATGACCGTTAGCGAATCGATGGCCG… 44.5 kD bacteriochlorophyll synthase subunit
1307_sll1905-sacB ATGTCCCCATCCCAGCCCCACCCAC… hybrid sensory kinase hik19
1308_slr1998-sacB ATGGAAGAGTTTGCAGTGGTGATAA… NAD(P)/FAD-dependent oxidoreductase
1309_slr1999-sacB ATGAAGGTTAAAGTAATCCTCAAAA… type II toxin-antitoxin system HicA family toxin
1310_slr2000-sacB ATGGCTCAAAAATCTTCGTCGTTTT… S-layer homology domain-containing protein
1311_slr2001-sacB ATGCCCCTATCCTCCCAACCGGCCA… cyanophycinase
1312_slr2002-sacB ATGAAAATTCTTAAAACCCTTACCC… cyanophycin synthetase cphA
1313_slr2003-sacB ATGGACACCACCTACCAAAAAAGTA… DUF4129 domain-containing protein
1314_sll1902-sacB ATGATTACTCCCCTTTCTTGGCAAG… glutathione S-transferase family protein
1315_ssr3402-sacB ATGAAGCAAATTATTCCCGCACTGA… hypothetical protein
1316_slr2004-sacB ATGCACCAAATATCAATGAAGCAAA… hypothetical protein
1317_slr1712-sacB ATGTTTGACAATCTGTGCAAATTTC… Rpn family recombination-promoting nuclease/putative transposase
1318_sll1615-sacB ATGCAATTAGAAGATACTATTGCGG… thiophen and furan oxidation protein thdF/mnmE
1319_sll1614-sacB ATGGATTTCCCCACCTTAAGTTCTT… cation-transporting ATPase; E1-E2 ATPase pma1
1320_sll1613-sacB ATGGCAGTGGCCAATACTAACTCCC… hypothetical protein
1321_sll1612-sacB ATGGATATCAACACCCTACTGGAAC… folyl-polyglutamate synthetase folC
1322_sll1611-sacB GTGCTCACTGCTCAATCTGAATACG… fatty acid desaturase
1323_slr1715-sacB ATGGCTTACAGTTTAGATTTAAGGC… transposase
1324_slr1716-sacB ATGTTAAGAGAACTAATTAAGCTCT… transposase
1325_sll1609-sacB ATGATGGTCGTTGCTACAACTAAAA… Uma2 family endonuclease
1326_sll1608-sacB ATGACTTTCTCCTTCCCCATGGCCC… DUF3488 domain-containing protein
1327_slr1717-sacB ATGTTAGAAAGCAAACTTGAAAATC… ATP-dependent sacrificial sulfur transferase LarE larE
1328_sll1606-sacB ATGTTCGTTTTTCTCTCCAAACTTT… YdcF family protein
1329_sll1605-codA ATGGCAATGTCAACTCCGGAGGTGA… (3R)-hydroxymyristol acyl carrier protein dehydrase fabZ
1330_slr1718-sacB GTGGAAATCTTTGTTTATCACACGC… 2-phosphosulfolactate phosphatase family protein
1331_slr1719-sacB ATGGACACCTTTGACGCTATTTACC… DrgA gene product drgA
1332_slr1720-sacB ATGCGTACTCATTATTGCGGCGACC… aspartyl-tRNA synthetase aspS
1333_slr1721-sacB ATGATGGAACGTTTTTCCCCTACTA… hypothetical protein
1334_slr1722-sacB GTGAACATTACGATTGGACGGGGAA… IMP dehydrogenase subunit guaB
1335_slr1723-sacB GTGAAACACTGGAAAACTTACCTAG… lactose transport system permease protein; LacG lacG
1336_sll1601-sacB ATGACGAAGCCATTGCCATATGACC… saccharopine dehydrogenase
1337_slr1724-sacB ATGTTATTTGCCCATGAGGCTTCCA… DUF3153 domain-containing protein
1338_ssl3127-sacB GTGGTGCTGGGGGGAGCTGACAGTA… branched-chain amino acid ABC transporter permease
1339_sll1600-sacB GTGAATCAGTTAGTGGTGGCCTTTC… Mn transporter; MntB mntB
1340_sll1599-sacB ATGGCAGCCACCCTATCTCGTCTTG… Mn transporter; MntA mntA
1341_sll1598-sacB ATGGCAACATCATTTGCTTCCCGGG… Mn transporter; MntC mntC
1342_ssr2898-sacB ATGCTCAGAACGGGGTGTGCCTGGC… transposase
1343_sll1596-sacB ATGGAAAATTTAAACGCTCTATCTG… KaiB-like protein 1
1344_sll1595-sacB ATGACAGATAACAGCCAAAGTCTCT… circadian clock protein KaiC kaiC
1345_slr1726-sacB ATGGCAGAAAAACTATCTTCCTCAC… DUF4230 domain-containing protein
1346_sll1594-sacB ATGCAAGCAACCTTACACCAATTAA… rubisco operon transcriptional regulator; LysR family regulator rbcR/ndhR
1347_slr1727-sacB GTGGATACAGCGGTCAACGAATCCC… eukaryotic Na+/H+ exchanger
1348_sll1592-sacB ATGGCAATTTCCCCAACTTCAATCC… NarL subfamily rre19
1349_sll1590-sacB GTGCGTATTTGGACCCGTACCCACT… sensory transduction histidine kinase
1350_slr1728-sacB ATGTTGCAAGGTTTTGTACAAATTG… potassium-transporting ATPase A chain kdpA
1351_slr1729-sacB ATGAACTCCACTTCCACTGTGCGAC… potassium-transporting ATPase B chain kdpB
1352_ssr2912-sacB GTGCCCTTTCCCCAGAGATTTTTCC… potassium-transporting ATPase subunit F kdpG
1353_slr1730-sacB ATGATTCGCAATTTTGTGATTAGTT… potassium-transporting ATPase C chain kdpC
1354_slr1731-sacB ATGATCCATGCCGGTCAATCCAGTT… potassium-transporting ATPase D chain kdpD
1355_sll1586-sacB ATGGTCACCGATCGCCAAAAGTCTG… DUF748 domain-containing protein
1356_sll0254-sacB ATGACTGAATTACCCCCCAATCCTA… FAD-dependent oxidoreductase
1357_sll0253-sacB GTGAAAACTGCGATGATCCCCATTT… hypothetical protein
1358_slr0260-sacB ATGGTCAATTCCCTGTCCGACGAGC… cob(I)alamin adenosyltransferase cobA
1359_ssl0467-sacB ATGAGCACCCAAGATAAAGCCCGTG… hypothetical protein
1360_sll0252-sacB ATGAAAAAATTAACGTCCTTTCCCT… SpoIID/LytB domain-containing protein
1361_slr0261-sacB ATGACCAAGATTGAAACCAGAACCG… NADH dehydrogenase subunit 7 ndhH
1362_slr0262-sacB ATGGCTAATGAGGTCGTTAAAGCTA… hypothetical protein
1363_slr0263-sacB ATGCGGGTAGAGAATATTACCCAAG… hypothetical protein
1364_slr0264-sacB ATGCGATCGCCTTTGTTGGCCCCGT… esterase
1365_slr0265-sacB ATGATAATTCGAGAATTTTACAGCA… transposase
1366_sll0250-sacB ATGGTAAAGGGGAAACGCATCCTAA… bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC dfp/coaBC
1367_ssl0461-sacB GTGAGTTGCCGACGGTTGTCAACTT… Cyanobacterial Signature Protein
1368_sll0249-sacB ATGACAAACCAGAATATGCCCCCGG… alpha/beta hydrolase
1369_sll0248-codA ATGACAAAAATTGGACTTTTTTACG… flavodoxin isiB
1370_sll0247-sacB GTGCAAACCTATGGCAACGACACCG… iron-stress chlorophyll-binding protein isiA
1371_slr0267-sacB ATGGCTTTAATCCAATGGTATCCCG… ribosome biogenesis GTPase YlqF ylqF
1372_sll0245-sacB ATGCTTAGAGCTGGAATTGTTGGTC… redox-regulated ATPase YchF ychF
1373_sll0244-sacB ATGGCCACGCAACAAACAATTTTGG… UDP-glucose 4-epimerase galE
1374_slr0269-sacB ATGCCATCTACTTTTTCCCAACCAT… hypothetical protein
1375_slr0270-sacB ATGATCAATCCCCAACCAGACGAAA… DUF2605 domain-containing protein
1376_ssl0453-sacB ATGATCAACAACGAAGCCTTTAACC… phycobilisome degradation protein; NblA nblA
1377_ssl0452-codA ATGAAACCTGAATCCTTCGATCTCA… phycobilisome degradation protein; NblA nblA
1378_slr0271-codA ATGCGAATCAACCATAAGCCTCTAT… hypothetical protein
1379_slr0272-sacB ATGACTACTCCCGTTTTTTGTACTA… zinc ribbon domain-containing protein
1380_slr0273-sacB ATGCCCAAAAATATGTCACCAATCT… hypothetical protein
1381_sll0243-sacB ATGATTGATTTTATTAAAAAAAAAG… zinc ribbon domain-containing protein
1382_sll0242-sacB ATGGACTTTGTCGAAGCCGAGCAAA… hypothetical protein
1383_sll0241-sacB GTGAAAATGAAACCATGGCGTTTTC… transcriptional regulator
1384_sll0240-sacB ATGATCAGAGTTGAACAGGTGAGCA… ABC transporter ATP-binding protein
1385_sll0238-sacB ATGCGATCGCCAACTAGCTTTGGGG… iron ABC transporter permease
1386_sll0237-sacB ATGCTTTTATCAACGAGGAAACAAT… extracellular solute-binding protein
1387_ssl0438-sacB ATGGGCATGACTATAATTTTGATAT… 50S ribosomal protein L12 honologue
1388_sll0236-sacB ATGACCGTTTCTCCCTTTGATTTCA… hypothetical protein
1389_slr2079-sacB ATGGGGATTGGCGTAAACTCAGATC… glutaminase
1390_ssr3532-sacB ATGGTTACAACTTTTAGCAAACCAT… hypothetical protein
1391_slr2080-sacB ATGCCAATCAGTGAAGATCTCAAAC… HNH endonuclease
1392_slr2081-sacB ATGAAAATTGGTGTTGTTGGTTTGG… prephenate dehydrogenase tyrA
1393_slr2082-sacB ATGACTAACCAGCCCACCGCATCGG… cytochrome c oxidase subunit I ctaD
1394_slr2083-sacB ATGGAATCAGGTAATCATCTCCCCC… cytochrome c oxidase subunit III ctaE
1395_slr2084-sacB ATGGCCGTTAATCTAGAAACTCGCA… Uma2 family endonuclease
1396_sll1988-sacB ATGGCAGACCAATTGATCCGCGCCA… Hsp33 family molecular chaperone HslO hslO
1397_sll1987-sacB ATGGGCACCCAACCCGCCAGGAAAT… catalase hpi katG
1398_slr2087-sacB ATGACCATTGCCAATCCTTCCCCTA… cytochrome c biogenesis protein ycf44
1399_slr2088-sacB GTGGTTGCATCAATTCCTAATCCCA… acetohydroxy acid synthase ilvG
1400_slr2089-sacB ATGGTTATTGCCGCCTCTCCCTCCG… squalene-hopene-cyclase shc