Plasmids and Cultures

This database contains entries for 3,572 known and predicted gene coding sequences in Synechocystis sp PCC 6803 based on the Kazusa genome sequence and nomenclature (http://genome.microbedb.jp/cyanobase/GCA_000009725.1). Plasmids for generating marked mutants and mutant strains can be searched for by gene Name/Accession (e.g. 'sll1226' or '1513_sll1226-sacB'),  gene homology (e.g. 'hydrogenase large subunit'), Gene ID (e.g. hoxH), or by a portion of the gene coding sequence Sequence (CDS).

Please note, the library is still being completed. Plasmids and/or marked mutants may not be available. Users can also search for available materials by Plasmids made or Mutant made.

Plasmids, mutants and the unmarking spacer plasmid pUC19K-UL are currently available on request. Regardless of the segregation status of the mutant, open reception of a cyanobacterial mutant strain we recommend 1-2 cycles of restreaking on a selection plate containing kanamycin (100 μg/ml).

Name Sort descending CDS Gene Homology Gene ID
3201_sll7033-sacB ATGAGTCGATTGTACGGTGACGAAA… hypothetical protein
3202_sll7034-sacB ATGACGAGTGCATTACAGGCGATCG… DUF433 domain-containing protein
3203_ssr7035-sacB GTGCGCCCAATCCAATCTGGGTCAC… hypothetical protein
3204_ssr7036-sacB ATGGAAACAGGGCACTCCATCGCCC… hypothetical protein
3205_slr7037-sacB ATGAAAGTTAGACCCCAGCACTGGC… DUF3854 domain-containing protein
3206_ssl7038-sacB ATGGAAACCCTTAAAACTATCCCTT… putative addiction module antidote protein
3207_ssl7039-sacB ATGATTGAAATCCGGCAAACAGAAA… type II toxin-antitoxin system RelE/ParE family toxin
3208_ssr7040-sacB ATGGAAGTCCGGCTCAAAAAGTGGG… AbrB/MazE/SpoVT family DNA-binding domain-containing protein
3209_slr7041-sacB ATGGTTATGACCTCAACCGTCCCGC… growth inhibitor PemK pemK
3210_ssl7042-sacB ATGGGCACTGCCACTAGATATACGG… DUF1819 family protein
3211_sll7043-sacB ATGCCTAAAAAACTATCAGTTCCTG… hypothetical protein
3212_sll7044-sacB GTGAAGATAGTCGTATTTAACGGCA… hypothetical protein
3213_ssl7045-sacB GTGGCTGCTAAACTCTCTTGCGCTG… hypothetical protein
3214_ssl7046-sacB ATGCAGGTGCTTACCTACCTCAAGT… putative toxin-antitoxin system toxin component%2C PIN family
3215_sll7047-sacB ATGACAAACCGAATGATAGAGACTT… DUF4386 domain-containing protein
3216_ssl7048-sacB ATGCTTGCTAAGTTGACATCTAAGA… AbrB/MazE/SpoVT family DNA-binding domain-containing protein
3217_slr7049-sacB ATGCTCATTGGTTATATGAGGGTTT… resolvase
3218_sll7050-sacB ATGCTTATTACCCTCGAGCCTACTT… hypothetical protein
3219_ssl7051-sacB GTGTTCGGAGGGGGCAACCATCTAT… hypothetical protein
3220_slr7052-sacB ATGGTTGCCCCCTCCGAACACCAGA… hypothetical protein
3221_ssl7053-sacB ATGTTGGAAGAAACGATCGCCGTAA… hypothetical protein
3222_slr7054-sacB GTGTATATACATTTGAAGAGGGGGC… DNA-binding protein
3223_sll7055-sacB GTGGGCTCTAGTTACCCCTTTACCT… hypothetical protein
3224_sll7056-sacB GTGATGGTGCTTATATTGAATAATT… hypothetical protein
3225_slr7057-sacB ATGACAAGGACTCTGCCACCTGTGT… HNH endonuclease
3226_slr7058-sacB ATGAAAGATGACCGTTATCCCTCCG… contain a COG3183 super family conserved domain
3227_slr7059-sacB ATGAGCACATTGCAACAATTCAGCC… MerR family transcriptional regulator
3228_slr7060-sacB ATGAATACGCCCCAAGTTAGTTTTA… VWA domain-containing protein
3229_slr7061-sacB ATGAAAATCATCTCTTTTCTCGGTT… TIGR02221 family CRISPR-associated protein
3230_sll7062-sacB ATGCCTAACGTTATTGTTTCCCCTT… hypothetical protein
3231_sll7063-sacB ATGACAGTCGGAACATTGGGCGTTG… TIGR03986 family CRISPR-associated RAMP protein
3232_sll7064-sacB ATGCCAGCAGGAGGCCGCCTAATGA… TIGR03984 family CRISPR-associated protein
3233_sll7065-sacB ATGGCTAGAAAAGTTACTACACGCT… hypothetical protein
3234_sll7066-sacB ATGCGAGGAATTGAGATAACCATAA… hypothetical protein
3235_sll7067-sacB ATGTTTCTAGTTCTAATTGAGACTT… hypothetical protein
3236_slr7068-sacB GTGGTGGATCTAAAATCCTTAGCTG… CRISPR-associated endoribonuclease cas8
3237_sll7069-sacB ATGAAAATCATTATTACTGCCAAGG… hypothetical protein
3238_sll7070-sacB ATGGAAAACCCTAATCAATACCAAA… hypothetical protein
3239_slr7071-sacB ATGACTAGTATTTACCTGACAGAAC… CRISPR-associated endonuclease cas1
3240_ssr7072-sacB ATGGATTTTTGGTTAGTTTGTTACG… CRISPR-associated endonuclease cas2
3241_slr7073-sacB ATGCCTAACCCCACCGTTGAAATTA… hypothetical protein
3242_ssl7074-sacB GTGTGCCAGGAAAACCCAGGTCATT… hypothetical protein
3243_sll7075-sacB ATGAGCATTTTACGATACGAAGAAA… hypothetical protein
3244_slr7076-sacB ATGTCGCTGGACACGCTGGTGCCGG… HNH endonuclease
3245_sll7077-sacB ATGAATTTATCCCATCCTACTCCCC… bifunctional (p)ppGpp synthetase/guanosine-3'%2C5'-bis(diphosphate) 3'-pyrophosphohydrolase
3246_sll7078-sacB GTGGATGCTAACTTTAATTTTCCTA… TIGR03985 family CRISPR-associated protein
3247_ssr7079-sacB ATGCAACGCAGAAGTACCCAAAGCC… No conserved domains
3248_slr7080-sacB ATGGTTAGTTATCATTTGCACCTGA… hypothetical protein
3249_slr7081-sacB ATGGTCAGCATTCACATTACCCGCC… hypothetical protein
3250_slr7082-sacB GTGGCTACCATTTCCCAACAAATTA… hypothetical protein
3251_slr7083-sacB ATGACAAGTCAAAATTTTGTTTCTG… hypothetical protein
3252_ssr7084-sacB ATGGCATCCCTTGTTGAAATCAAAG… hypothetical protein
3253_sll7085-sacB GTGGAATATCTGCGCTGGATGCGTC… hypothetical protein Cmr6-Cmr1 fusion protein
3254_sll7086-sacB ATGCCAAACTTTGACCCCCGTACCA… hypothetical protein Cmr5
3255_sll7087-sacB TTGACTGTTCTCTTATTATCAATTT… type III-B CRISPR module RAMP protein Cmr4
3256_slr7088-sacB ATGGATTTAGCTGTTAGTAAGATTC… peptidase
3257_sll7089-sacB ATGTATTGGTATAGCCTCACTCCCC… hypothetical protein Cmr3
3258_sll7090-sacB GTGTCTGAAGTTTACTGGCAAGCAA… type III-B CRISPR-associated protein Cas10/Cmr2
3259_slr7091-sacB GTGTTTAAAAAACTTCATACTATTT… hypothetical protein
3260_slr7092-sacB GTGAAAACTCTCTATGTTTCCCAAT… CRISPR-associated endonuclease Cas1
3261_ssr7093-sacB ATGTTTCTCTATGTTATTGCCTACG… CRISPR-associated endonuclease Cas2
3262_slr7094-sacB ATGAATAGTATTTTCACCATTAACC… hypothetical protein
3263_slr7095-sacB ATGACATCTGTTTTTCACGTTCTAA… adenylyl-sulfate kinase
3264_slr7096-sacB GTGAGATATAAAAATAATTGTCGAA… hypothetical protein
3265_slr7097-sacB TTGATTAAACAACTCAGCCTTTTTG… alpha-ketoglutarate-dependent dioxygenase alkB
3266_slr7098-sacB ATGTACGACGCGCTATTGCCTATAG… hypothetical protein
3267_slr7099-sacB ATGACTGATTTCAAAGCATTGATTG… hypothetical protein
3268_slr7100-sacB ATGACCATCAACACCGATGCCATTC… hypothetical protein
3269_slr7101-sacB ATGATGATAATTTGCTGGAGGATGA… hypothetical protein
3270_slr7102-sacB ATGAAAAAATTACTCAAATCGCTCA… hypothetical protein
3271_sll7103-sacB ATGCAACTAAAAAATGATTATCATC… exodeoxyribonuclease V alpha chain
3272_slr7104-sacB ATGGCTTACAGTTTAGATTTAAGGC… transposase
3273_slr7105-sacB ATGTTAAGAGAACTAATTAAGCTCT… transposase
3274_sll8049-sacB type I site-specific deoxyribonuclease chain R
3275_sll8001-sacB ATGTTTGAATGTAAAAGTGGCAACA… Appr-1-p processing protein
3276_sll8002-sacB ATGACCGTTCCCGCTCAGCCTAAGA… DUF4433 domain-containing protein
3277_ssl8003-sacB ATGACCAATCAACAGGGTAATGTTA… hypothetical protein
3278_sll8004-sacB ATGTGGAACGGGAGAGAATATATGT… hypothetical protein
3279_ssl8005-sacB ATGAAACGCCAATCTGTCCTGCAAT… DNA polymerase subunit beta
3280_sll8006-sacB ATGAAACTGAAGCCCTATCCCGAAT… type I restriction-modification system S subunit
3281_sll8007-sacB ATGCGAATCCTGCTTGATGAATGCG… hypothetical protein
3282_ssl8008-sacB ATGGTGACCACAGCCAGAGTAATGC… DUF433 domain-containing protein
3283_sll8009-sacB GTGCAAGACATAGATGGTAACGAGG… type I restriction-modification system M subunit
3284_ssl8010-sacB ATGAACCCAGTTAGCCACAACAACA… type I restriction-modification system M subunit
3285_sll8011-sacB ATGTTAGGTTTTCTATTGGATGAAC… hypothetical protein
3286_sll8012-sacB ATGTCCATAGTAGTCAGTACCCGTC… hypothetical protein
3287_ssr8013-sacB ATGACAGCGCAAGAAAAACTCTACC… DUF2281 domain-containing protein
3288_slr8014-sacB GTGAAAGTACTTCTCGACACCAATA… PIN domain-containing protein
3289_slr8015-sacB ATGTCAAAAACCATAGCCATAGTCA… ParA family plasmid partitioning protein
3290_slr8016-sacB ATGGCAAAACGTCAATCTCTAGACA… plasmid partitioning protein
3291_sll8017-sacB GTGACAGAATTGGCCCAGGAAGCCG… hypothetical protein
3292_sll8018-sacB GTGGTCATGGGGATAATCAAGAATC… hypothetical protein
3293_sll8019-sacB GTGCTAACGACCAAGCATCTGCCCC… hypothetical protein
3294_sll8020-sacB ATGAGCCTCCCCGACAATGTCACCA… Crp/Fnr family transcriptional regulator
3295_slr8021-sacB ATGACGGTCACTCCACTCAATCTTA… class I SAM-dependent methyltransferase
3296_slr8022-sacB ATGAAAACCCCAATCCTTTCGTTTC… cupin domain-containing protein
3297_slr8023-sacB ATGAAAGCAAGCTTTGGCACTGAGA… carboxylesterase family protein
3298_ssl8024-sacB GTGCAACATCATATTTGCTATTGTT… No conserved domains
3299_sll8025-sacB ATGAGATTAAAGATTAGTAAGAGAA… hypothetical protein
3300_slr8026-sacB ATGCAAATTTTTCCAAGGCGAGAGG… MarR family transcriptional regulatory protein
3301_sll8027-sacB ATGAAGGCTTCTCCACGGGTTGTTA… putative toxin-antitoxin system toxin component%2C PIN family
3302_ssl8028-sacB ATGCTGGCTAAGTTGACATCTAAGA… AbrB/MazE/SpoVT family DNA-binding domain-containing protein
3303_slr8029-sacB ATGGCGATCGTCGGTTATGCACGGG… resolvase
3304_slr8030-sacB ATGGTCTGGTCACGTCGAAATTTTC… DUF839 domain-containing protein
3305_sll8031-sacB ATGTCCACCAGCACCCATGCCCTCA… NADH dehydrogenase subunit ndhK2
3306_sll8032-sacB ATGAGTAACCTTATTCTGCAAATCA… hypothetical protein
3307_sll8033-sacB ATGCGAGCCTTACCTTCTTTCCCCA… hypothetical protein
3308_sll8034-sacB ATGCTAACCACCCAAATCACCCAAA… 2-nitropropane dioxygenase
3309_sll8035-sacB ATGTTTGCCAACGTGATCGCCACCC… hypothetical protein
3310_slr8036-sacB GTGCTGTCTCTAGGGCAAATCTATG… GNAT family N-acetyltransferase
3311_slr8037-sacB GTGTTTCGATGGTTTATCGCTGCGA… GNAT family N-acetyltransferase
3312_slr8038-sacB ATGCCGTTTGAAGATGCCCTTGCGA… WD-repeat protein
3313_ssl8039-sacB ATGGAAGGTTTACAAGAAGAATCTA… no conserved domains
3314_sll8040-sacB ATGGAAATACTTACACGGTACAACT… HNH endonuclease
3315_ssl8041-sacB ATGAAATTATCCGTTCTTACTGTGG… transposase
3316_sll8042-sacB ATGTTAAGAGAACTAATTAAGCTCT… transposase
3317_sll8043-sacB ATGGCTTACAGTTTAGATTTAAGGC… transposase
3318_slr8044-sacB ATGGGATCTATGACCGCCGAAGAAG… hypothetical protein
3319_slr8045-sacB ATGGCTTACAGTTTAGATTTAAGGC… transposase
3320_slr8046-sacB ATGTTAAGAGAACTAATTAAGCTCT… transposase
3321_ssr8047-sacB ATGTCATTGGATAGACAATTAAACC… hypothetical protein
3322_sll8048-sacB ATGAATACTAGCATCACCACCACTC… M48 family peptidase
3323_sll5132-sacB DUF262 domain-containing protein
3324_ssl5001-sacB ATGAATACCGTAACCGCCGTAAGAG… no conserved domains
3325_sll5002-sacB ATGCCTGACATTCAAACTTGGGAAT… hypothetical protein
3326_sll5003-sacB GTGATCGAGCTTACCAACCCCTTTA… hypothetical protein
3327_sll5004-sacB GTGAATATGTTAGGTCGCCACTCTG… DUF433 conserved domains
3328_slr5005-sacB ATGGCCAATCCTTATGCCTCCTATG… DUF4114 domain-containing protein
3329_sll5006-sacB ATGTCCATCGCTGGAAAGTCCAAAG… hypothetical protein
3330_ssl5007-sacB ATGGACTTTAACCCCAATGAGTGGC… hypothetical protein
3331_ssl5008-sacB GTGGATGTCCGCCCATGGCACCAGA… no conserved domains
3332_ssr5009-sacB GTGACAACTGTTACCGATGCAGACC… hypothetical protein
3333_slr5010-sacB ATGAAAATTAACCGTCAGGGGCAGG… hypothetical protein
3334_ssr5011-sacB ATGAAAAAACAAGTTAAAGTCAAAC… DUF167 domain-containing protein
3335_slr5012-sacB ATGAAAGTTCAGCTTGGTCTTGATA… DUF4007 family protein
3336_slr5013-sacB GTGACTACACTCAGCCAATATTTTC… hypothetical protein
3337_sll5014-sacB ATGAGTCAAATATTTCAATGTCCTT… hypothetical protein
3338_ssl5015-sacB ATGAGTGATTTCCCAAAAGACTGGG… hypothetical protein
3339_slr5016-sacB ATGGGAAAAGCAGGACAAGCATTAA… hypothetical protein
3340_slr5017-sacB ATGCTCTCAGTAACTCACCTCAAAA… DNA polymerase subunit beta
3341_slr5018-sacB ATGAATCGTGACAACGCTTATCTGC… DUF86 domain-containing protein
3342_ssr5019-sacB ATGCAAAGCAATAAGCAGATCCACC… aminotransferase class V-fold PLP-dependent enzyme
3343_ssr5020-sacB GTGATTCGTAACTTTAAAGATAAAG… hypothetical protein
3344_slr5021-sacB ATGAATCCAGACAAACTAAAACCTA… addiction module antidote protein%2C HigA family higA
3345_slr5022-sacB TTGATCTACCTCGATTACCATGCCA… cysteine desulfurase
3346_slr5023-sacB ATGAAAATTGTTTCTATAAAAATAA… chromosome segregation protein SMC
3347_slr5024-sacB ATGAGTTACGACTTAATTTTTCTTC… DUF4276 family protein
3348_ssl5025-sacB ATGCTACAGAACTTAGATCGAATCA… DUF433 domain-containing protein
3349_sll5026-sacB ATGGCGGATACTCATAATTTTATTG… DNA phosphorothioation-associated putative methyltransferase
3350_ssl5027-sacB ATGAAGAATAATCGTTATTCTCCTG… HNH endonuclease
3351_sll5028-sacB GTGGGAAGCAGTATTGAATCCGTTG… hypothetical protein
3352_slr5029-sacB ATGACAAAGACAAGAGAGGCAAAAA… transposase
3353_sll5030-sacB ATGAAAACCTCTTTAGATTGTATTC… DUF89 family protein
3354_ssl5031-sacB GTGCACGTTTCTTTGAATGTGGTTC… DUF4079 super family conserved domain
3355_sll5032-sacB ATGAACTCAGAAAATCTCGCGGCTC… DUF4079 domain-containing protein
3356_sll5033-sacB ATGAAAAGCATCTTAAGTTTTATCA… DUF3122 domain-containing protein
3357_sll5034-sacB ATGCAAACTTTACCACAACGGATTT… DUF3365 domain-containing protein
3358_sll5035-sacB ATGCAAATATCTGTGAATAAAACCC… ArsR family transcriptional regulatory protein
3359_sll5036-sacB ATGGCGCAGATTGTCGTAATTGGAG… sulfide-quinone reductase
3360_slr5037-sacB ATGTATTACTTTAGTAAAAAATTGC… DUF302 domain-containing protein
3361_slr5038-sacB ATGAAAGAAATACATAGCTTAGAAA… chromate transporter
3362_ssl5039-sacB ATGGATCAAAACGAGTGGGTTATCT… hypothetical protein
3363_slr5040-sacB ATGCTCAGAACGGGGTGTGCCTGGC… transposase
3364_sll5041-sacB GTGGATACCTTACTAGACGAGTTGC… transposase
3365_sll5042-sacB ATGAAATGTGCCACCAGTACCCTCC… sulfotransferase
3366_sll5043-sacB ATGGCCATAATTGCTTCAAACAATA… glycosyltransferase family 2 protein
3367_sll5044-sacB ATGATCCAAGAGTTTCAGCGGTTTA… hypothetical protein
3368_ssl5045-sacB GTGTTTGAAAAGCCCCCCTGGTCCC… no conserved domains
3369_sll5046-sacB ATGAACACTTCTTTCCTCGAATCCC… sulfotransferase
3370_sll5047-sacB ATGACCCCCCAAGCCCAACTGGTTC… hypothetical protein
3371_sll5048-sacB ATGAAAATCACCTTTGTTTTACCCT… glycosyltransferase
3372_sll5049-sacB ATGGCTTCCCTCAATAAACTGGCCA… hypothetical protein
3373_sll5050-sacB ATGGTGAGATCGCCCCAATTCCGGG… colanic acid biosynthesis glycosyltransferase wcaL
3374_slr5051-sacB ATGCCAACAGTTGAAACTGACTTGG… hypothetical protein
3375_sll5052-sacB ATGGCTCACGATGAAGCAGAAGAAT… hypothetical protein
3376_slr5053-sacB ATGGTGGCGATCGCCAAACTTAGTG… cyanoexosortase A system-associated protein
3377_slr5054-sacB ATGGAAGATTTAGGAATTGTCGTTA… glycosyltransferase
3378_slr5055-sacB ATGAATTCCATCACGCTTCTCAACA… glycosyltransferase
3379_slr5056-sacB GTGTTTCGGGGTTGCCTAGCCAAAG… glycosyltransferase
3380_sll5057-sacB ATGGCTAGCACTATTCAACTGATTG… STAS domain-containing protein
3381_slr5058-sacB ATGGCAAAACGTTCCCTTAAAGCTT… NACHT domain-containing NTPase xssQ
3382_sll5059-sacB ATGACTGAGACTTCTCCGACGGTTG… two-component response regulator xssR
3383_sll5060-sacB GTGATTAATTCGGGAACCTTAATTA… two-component hybrid sensor and regulator xssS
3384_sll5061-sacB ATGATAACCCTGACCAGTCCTGGAG… PAS domain S-box protein
3385_sll5062-sacB ATGGGGAGTTTTGGGCTATTGTTCA… hypothetical protein
3386_sll5063-sacB GTGATAGCCTTGCTTCAGCGAGGGG… hypothetical protein
3387_ssl5064-sacB ATGAAAGATAACCGGTATTCTCCTG… HNH endonuclease
3388_ssl5065-sacB ATGGGACGGCTCAGTAAGGTGTTTG… hypothetical protein
3389_sll5066-sacB ATGAAGATAATCGCCGTGACTGGCT… ParA family protein
3390_sll5067-sacB ATGCTCAAAACGGCCTATATTGTCT… ATP-binding protein
3391_ssl5068-sacB GTGAGGAGTTCTAGCCATGGAACCC… DNA cytosine methyltransferase
3392_sll5069-sacB ATGACTCAATTCTACAAGCTAACAA… hypothetical protein
3393_ssl5070-sacB GTGGTTTTACATGGGAAAATATATT… no conserved domains
3394_slr5071-sacB ATGGATGACAGGGATCTAGTAAAAA… XRE family transcriptional regulator
3395_sll5072-sacB ATTGGCGATCGCCTCACCCTCACCG… no conserved domains
3396_slr5073-sacB ATGATTGTTGAACGGTGCTATTTTC… hypothetical protein
3397_ssr5074-sacB ATGTTTAAATTTATAGGCATTGTAT… hypothetical protein
3398_sll5075-sacB ATGAAAGAAGACTTTGGTTTTACAC… VOC family protein
3399_sll5076-sacB ATGAAAGCTACTGTATATATTGCAA… dihydrofolate reductase
3400_slr5077-sacB ATGTTTGATAATCTGTGCAAATTTC… Rpn family recombination-promoting nuclease/putative transposase